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Proliferative response of T cells from tumor‐immune chickens to carcinogen‐induced fibrosarcoma cells
Author(s) -
Quere Pascale,
Tsiagbe Vincent K.,
Thorbecke G. Jeanette
Publication year - 1990
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910450628
Subject(s) - fibrosarcoma , spleen , immune system , biology , carcinogen , cellular immunity , immunology , microbiology and biotechnology , cancer research , pathology , medicine , genetics
Immunity to carcinogen‐induced transplantable fibrosarcomas (CHCT‐NYU‐4, ‐97, ‐36 and ‐20) in SC chickens was studied by the ability of spleen cells from NYU‐4 or ‐97 immune chickens to proliferate in response to tumor cells in vitro . Spleen, but not peripheral blood cells, from NYU‐4 immune chickens proliferated significantly more vigorously to gamma‐irradiated NYU‐4 cells than did cells from normal chickens. The proliferative response was not much affected by addition of indomethacin. Spleen cells from NYU‐4‐immune agammaglobulinemic (Aγ) chickens exhibit the same ability to proliferate in presence of gamma‐irradiated NYU‐4 tumor cells. Analysis of the phenotype of the T‐cell component involved in proliferation showed that the proliferative response was significantly decreased by removal of CT4 + cells through indirect panning. Removal of CT8 + cells enhanced background proliferation without affecting the total thymidine incorporation in the presence of tumor cells. Immune spleen cells usually gave highest responses to the immunizing tumor, but also exhibited cross‐reactivity to cells from other individual tumors induced by the same carcinogen.