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c‐fos transfection of 3LL tumor cells turns on MHC gene expression and consequently reduces their metastatic competence
Author(s) -
Kushtai G.,
Feldman M.,
Eisenbach L.
Publication year - 1990
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910450624
Subject(s) - transfection , microbiology and biotechnology , biology , messenger rna , clone (java method) , gene , c fos , gene expression , cell culture , cell , cancer research , genetics
Transfection with c‐ fos genes of cells of a highly metastatic (H‐2K − H‐2D + ) clone, D 122, of the 3LL carcinoma, causes activation of H‐2K gene expression. Experiments were carried out to test whether these transfectants exhibit reduced metastatic competence. Studying 11 mouse c‐ fos , 6 mouse c‐ fos and 2 v‐ fos transfected clones, we observed that clones expressing high steady‐state levels of the fos mRNA also expressed elevated levels of H‐2K and H‐2D mRNA, and high levels of cell‐surface H‐2K and H‐2D glycoproteins. The transfectants were tested for generation of spontaneous metastasis following intra‐footpad inoculation of the tumor cells. Clones expressing high levels of fos and of H‐2 antigens, particularly those expressing high levels of cell‐surface H‐2K b molecules, showed a reduction of their metastatic competence. Statistical analysis revealed that c‐ fos transfectants are significantly less metastatic than the parental cells. The molecular mechanisms of c‐ fos activation of H‐2 genes is briefly discussed.