Premium
Inhibitory effect of quercetin on the synthesis of a possibly cell‐cycle‐related 17‐kDa protein, in human colon cancer cells
Author(s) -
Hosokawa Nobuko,
Hosokawa Yohei,
Sakai Toshiyuki,
Yoshida Mitsunori,
Marui Nobuyuki,
Nishino Hoyoku,
Kawai Keiichi,
Aoike Akira
Publication year - 1990
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910450622
Subject(s) - cancer , cell cycle , colorectal cancer , cancer research , cell , quercetin , inhibitory postsynaptic potential , biology , cancer cell , protein expression , medicine , biochemistry , chemistry , endocrinology , genetics , gene , antioxidant
Abstract Quercetin inhibits growth of COLO320 DM cells, derived from a human colon cancer. The inhibitory effect is partially reversible when quercetin is removed from the culture medium. Flow cytometric analysis has revealed that quercetin causes perturbation of the cell cycle, inducing a frozen cell‐cycle pattern and a block at the G 1 /S boundary. The synthesis of a 17‐kDa protein was specifically inhibited by the addition of quercetin, and recovered when the cells at the G 1 /S boundary progressed into S‐phase after the removal of quercetin from the culture medium. Furthermore, using synchronized cells obtained by centrifugal elutriation, we have shown that the rate of synthesis of a 17‐kDa protein was low in G 1 , and high in S‐phase of the cell cycle. Thus, this protein appears to be cell‐cycle‐related.