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Establishment and characterization of a leukemic murine cell line derived from MCF 247 MuLV‐induced T‐cell lymphoma
Author(s) -
Dolcetti Riccardo,
Carbone Antonino,
Barlati Sergio,
Maestro Roberta,
Rizzo Silvana,
Sonego Fulvia,
Feriotto Giordana,
Bellotti Daniela,
Boiocchi Mauro
Publication year - 1990
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910450525
Subject(s) - lymphoma , cell culture , leukemia , cancer research , biology , in vivo , murine leukemia virus , cell , plasminogen activator , immunology , genetics
Understanding of the leukemic evolution of human non‐Hodgkin's lymphomas is hindered by the lack of appropriate animal models. For this purpose, a highly leukemic cell line NQ22, derived from a MCF 247 murine leukemia virus (MuLV)‐induced murine T‐cell lymphoma, was established, and its preliminary characterization is described. The NQ22 cell line is easily transplantable subcutaneously (s.c.) into syngeneic AKR mice exhibiting early peripheral blood invasion and widespread dissemination with a leukemic pattern of infiltration. Such peculiar in vivo behavior is a stable phenotypic feature, probably determined genetically. Biological and differentiation characteristics of the NQ22 cell line were analyzed and compared to those of other non‐leukemic T‐lymphoma lines. In addition, no evidence of possible involvement of plasminogen activator (PA) enzymes and of their inhibitors (PA1) in the spreading ability of NQ22 cells was observed.