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Differences in C‐ myc and pvt ‐1 amplification in sewa sarcoma sublines selected for adherent or non‐adherent growth
Author(s) -
Minarovits Janos,
Steinitz Michael,
Boldog Ferenc,
Imreh Stephan,
Wirschubsky Zvi,
Ingvarsson Sigurdur,
Hedenskog Mona,
MinarovitsKormuta Susanna,
Klein George
Publication year - 1990
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910450324
Subject(s) - sarcoma , biology , ascites , in vivo , cancer research , phenotype , neoplasm , microbiology and biotechnology , cell , in vitro , solid tumor , cancer , pathology , genetics , gene , medicine
Conversion of solid sarcomas and carcinomas into ascites tumors depends on the in vivo selection of phenotypically altered tumor cell variants that can grow in the dissociated form. Once selected, they retain this property even after prolonged s.c. growth as solid tumors. From an s.c.‐passaged subline of an ascites‐converted murine sarcoma (SEWA‐AS12), we were able to separate cells adapted to the ascites form of growth from cells that can only grow in the solid form on the basis of their differential adherence to plastic. Both c‐ myc and pvt ‐1 were amplified approximately 63‐ to 77‐fold in the nonadherent subline (SEWA‐AS12‐NA), but only 5‐ to 8‐fold in the adherent subine (SEWA‐AS12‐ADH). This suggests that c‐ myc and/or pvt ‐1 amplification may provide a selective advantage to cells that can grow in the dissociated form.