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Rat monoclonal antibodies to the external domain of the product of the C‐ erb B‐2 proto‐oncogene
Author(s) -
Styles Jennifer M.,
Harrison Shona,
Gusterson B. A.,
Dean C. J.
Publication year - 1990
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910450219
Subject(s) - monoclonal antibody , antibody , epitope , microbiology and biotechnology , oncogene , antigen , biology , breast carcinoma , cancer research , chemistry , cancer , immunology , cell , breast cancer , biochemistry , cell cycle , genetics
With the breast carcinoma cell line BT 474 used as a source of antigen, four rat monoclonal antibodies (MAbs) (3 lgG 2a and I IgA) have been prepared against the external domain of the product of the c‐erb B‐2 proto‐oncogene. All 4 antibodies stain frozen sections of tissues that over‐express the product of the c‐erb B‐2 proto‐oncogene, and competitive binding assays showed that the antibodies recognized 2 non‐overlapping epitopes. Representative antibodies from the two groups (ICR12 and 13) were shown to specifically immunoprecipitate a 190kDa protein from 35 S‐methionine‐labelled breast carcinoma cells where the c‐erb B‐2 is amplified (BT 474 and MDA‐MB 361). Two of the antibodies (ICR12 and ICR17) bind to the denatured antigen in Western blots and ICR 12 stains formolsaline‐fixed sections of breast carcinoma tissue that overexpresses the product of the c‐erb B‐2 proto‐oncogene. These antibodies should be useful not only for immunocytochemical diagnoses but also for radio‐immunoscintigraphic and therapeutic applications.