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Oncofetal antigen in Xiphophorus detected by monoclonal antibodies directed against melanoma‐associated antigens
Author(s) -
Claus Gert,
Winkler Christoph,
Lohmeyer JÜRgen,
Anders Fritz,
Schartl Manfred
Publication year - 1990
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910450125
Subject(s) - monoclonal antibody , antigen , immunoperoxidase , biology , oncofetal antigen , melanoma , immunofluorescence , antibody , microbiology and biotechnology , immunohistochemistry , pan t antigens , epitope , immunology , pathology , cancer research , medicine , tumor associated antigen
Monoclonal antibodies (MAbs) directed against Xiphophorus melanoma cells were developed and tested by indirect immunofluorescence and immunoperoxidase staining for reactivity with a panel of 15 allogeneic tissues and 12 allogeneic cell lines. The reactivity of such MAbs was restricted to melanoma cells from tumor biopsies and melanoma‐derived cell lines. In addition, all embryonic cells of all histiotypes from developmental stages later than mid‐organogenesis and from corresponding short term in vitro cultures reacted with these MAbs. In contrast, normal tissues and organs from adult fish displayed no reactivity, thus implying that the melanomaassociated antigens detected by the MAbs described are oncofetal antigens.