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The effect of the E 2 prostaglandin enprostil, and the somatostatin analogue sms 201 995, on the growth of a human gastric cell line, MKN45G
Author(s) -
Watson Susan A.,
Durrant Lindy G.,
Morris David L.
Publication year - 1990
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910450117
Subject(s) - gastrin , endocrinology , medicine , somatostatin , in vivo , in vitro , cell culture , cell growth , peptide hormone , hormone , biology , biochemistry , genetics , microbiology and biotechnology , secretion
The effect of enprostil and the somatostatin analogue SMS 201 995 on the growth of a clonal variant of the human gastric adenocarcinoma cell line, MKN45, was studied. The derived cell line grew twice as fast as MKN45 when grown as a xenograft line in nude mice. However, it did not respond trophically to gastrin either in vitro or in vivo (unlike MKN45) although it possessed the same number of gastrin receptors as the parental line. Gastrin production by the cell line during in vitro culture was twice that of MKN45; thus, the cell line was denoted MKN45G. When MKN45G was grown as xenografts in nude mice (n =10/group), enprostil (20 μg/kg/day) significantly inhibited tumour growth when administered continuously by an osmotic mini‐pump from day 1 to day 7 of a 20‐day experiment, and induced tumour regression when administered from day 7 to day 14. Enprostil reduced post‐prandial serum gastrin levels when administered from day 7 to day 14 and prevented gastrin release by MKN45 in vitro. SMS 201 995 at doses of 25 and 240 μg/kg/day induced tumour regression when administered from day 1 to day 7 and the former dose reduced post‐prandial serum gastrin levels at day 5. Gastrin release by MKN45G was not affected by SMS 201 995 in vitro , thus its effect may not be mediated directly via gastrin, requiring interaction between other hormones or growth factors in the in vivo situation.