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Studies on the oncogenic potential of epstein‐barr‐virus (EBV)‐infected B cells in aids‐related disorders
Author(s) -
Roncella Silvio,
Caretto Patrizia,
Forni Guido,
Cutrona Giovanna,
Verde Antimo,
Ramarli Dunia,
Celle Paola Francia Di,
Foà Robin,
Sessarego Mario,
Pistoia Vito,
Ferrarini Manlio
Publication year - 1989
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910440721
Subject(s) - biology , virus , karyotype , phenotype , epstein–barr virus , cell culture , virology , cloning (programming) , lymphoblast , cell , cancer research , microbiology and biotechnology , immunology , genetics , chromosome , gene , computer science , programming language
Spontaneous lymphoblastoid cell lines (LCLs) were established from the peripheral blood of 10 human immunodeficiency virus (HIV)‐seropositive patients in order to investigate whether or not progression of the cells towards a malignant state could be traced. The LCLs studied displayed no differences in their surface phenotype, karyotype, and tumorigenicity in nude mice as compared with a wide panel of control LCLs. Furthermore, no c‐myc rearrangement could be detected in any of the LCLs. However, 4 of the 10 LCLs derived from HIV‐seropositive patients formed colonies in agar with a cloning efficiency of 0.1–0.9%. This percentage was much lower than that of a control neoplastic B cell line (50%), but consistently higher than that observed for a battery of spontaneous LCLs. The cells of a number of sublines that were derived from the agar colonies expressed new activation markers (CD10 and Bac‐I) but did not induce tumors in nude mice or display chromosomal abnormalities. These sublines might comprise cells that have progressed towards a more markedly transformed state.