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Clustering of discrete cell properties essential for tumorigenicity and metastasis. II. Studies of syrian hamster embryo fibroblasts transformed by rous sarcoma virus
Author(s) -
Deichman G. I.,
Kashleva H. A.,
Kluchareva T. E.,
Matveeva V. A.
Publication year - 1989
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910440527
Subject(s) - rous sarcoma virus , hamster , in vivo , in vitro , biology , embryo , serial passage , microbiology and biotechnology , cell culture , virology , virus , phenotype , biochemistry , genetics , gene
Tumorigenic and metastasizing activities (TGA; MA) and susceptibility, or resistance to H 2 0 2 and PGE‐releasing activity (H 2 O R 2 + PGE s+ phenotype) have been examined in 6 Syrian hamster embryo cell strains transformed in vitro with Rous sarcoma viruses (Schmidt‐Ruppin and Prague strains). Early observations of extremely high level of TGA and even MA of RSV‐SR‐transformants never selected in vivo have been confirmed. The correspondence of these properties with a high level of expression of H 2 O R 2 + PGE s+ phenotype and its clustering character were demonstrated in 4 RSV‐SR trans‐formants, while significantly lower expression of all these characteristics, including TGA, was observed in 2 RSV‐Prague transformants. High level of spontaneous MA was noticed in some RSV‐SR transformants. A tumor cell line induced in vivo by RSV‐SR did not differ from the cell strain transformed in vitro by RSV‐SR. inhibition of H 2 O R 2 + PGE s+ phenotype in one of RSV‐SR transformants was obtained with non‐toxic doses of BCNU and indomethacin, leading to a marked decrease of TGA.