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Clustering of discrete cell properties essential for tumorigenicity and metastasis. I. Studies of syrian hamster embryo fibroblasts spontaneously transformed in vitro
Author(s) -
Deichman G. I.,
Kluchareva T. E.,
Matveeva V. A.,
Kushlinsky N. E.,
Bassalyk L. S.,
Vendrov E. L.
Publication year - 1989
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910440526
Subject(s) - in vivo , hamster , biology , in vitro , cell , population , cytotoxicity , cytotoxic t cell , cell culture , embryo , microbiology and biotechnology , phenotype , secretion , immunology , genetics , biochemistry , gene , medicine , environmental health
The expression of two discrete cell properties related to the host natural effector mechanisms, i.e. , resistance to damage by H 2 0 2 , a cytotoxic product of activated macrophages, and the ability to secrete PGE, which inhibits NK‐cell cytotoxicity, has been examined in parental Syrian hamster embryo cells spontaneously transformed in vitro (STHE strain) and in 18 in vivo selected sublines. In all cell variants, resistance to H 2 0 2 and PGE‐releasing activity were either both expressed, or not expressed at all. Parental STHE cells and 5 variants selected in vivo , which were equally highly susceptible to H 2 0 2 ‐induced damage, did not release any detectable amount of PGE upon contact with NK cells. In contrast, 13 other STHE variants selected in vivo and characterized by their resistance to H 2 0 2 , all released PGE upon contact with NK cells. Thus, these two biochemically unrelated cell phenotypic characteristics are likely to be either simultaneously selected in vivo , or united in cluster which pre‐exist or appear in rare cell variants of the parental cell population in the conditions of in vivo natural selection pressure.