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Monoclonal antibody against human squamous‐cell‐carcinoma‐associated antigen
Author(s) -
Tatake Revati J.,
Amin Kishore M.,
Maniar Hina S.,
Jambhekar Nirmala A.,
Srikhande Sumati S.,
Gangal Sudha G.
Publication year - 1989
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910440516
Subject(s) - monoclonal antibody , pathology , antigen , immunofluorescence , adenocarcinoma , cell culture , microbiology and biotechnology , carcinoma , biology , epidermoid carcinoma , antibody , cell , cancer , cancer research , medicine , immunology , genetics
Mouse monoclonal antibody (MAb) 3F8E3 (lgG 3k ) was developed against the head and neck cancer cell line LICR‐LON‐HN2. Subjected to indirect immunofluorescence, the MAb reacted exclusively with SCC cell lines and showed no reactivity with normal or transformed mouse and human non‐SCC cell lines and hematopoietic cell lines. The radiolabeled MAb showed an affinity constant of 1.8 × 10 8 M −1 with HN2 cells and identified 2.07 × 10 4 sites/cell by Scatchard analysis. It identified 2 peptides from membrane extracts of HN2 cells by Western blotting. Avidin‐biotin‐complexed im‐munoperoxidase staining on cryostat sections of tumors from various tissues revealed that 3F8E3 reacted mainly with the membrane antigens of well differentiated SCC cells of oral cavity, larynx, esophagus, lung, uterine cervix, metastatic nodes of patients with oral cancer, and dysplastic cells in oral leukoplakia. The MAb did not react with poorly differentiated cells of Ca esophagus, adenocarcinoma of breast, stomach and colon, renal‐cell carcinoma and soft‐tissue sarcoma.