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IL‐1 and IL‐6 release by tumor‐associated macrophages from human ovarian carcinoma
Author(s) -
Erroi Annalaura,
Sironi Marina,
Chiaffarino Francesca,
ZhenGuo Chen,
Mengozzi Manuela,
Mantovani Alberto
Publication year - 1989
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910440508
Subject(s) - monokine , ovarian carcinoma , cytokine , endocrinology , medicine , radioimmunoassay , lymphokine , ascites , carcinoma , interleukin , immunology , cancer research , biology , immune system , ovarian cancer , cancer
Our study was designed to investigate the production of interleukin‐l (IL‐I) and IL‐6 in tumor‐associated macrophages (TAM) isolated from ascites (18 cases) or solid (7 cases) human ovarian carcinoma. These are pleiotropic monokines which, in addition to affecting proliferation and differentiation of lymphocytes, act on various targets, including vascular cells and liver, and may therefore be involved in the pathogenesis of certain manifestations of malignancy. IL‐I was measured by the thymocyte costimulator assay, under conditions in which IL‐6 was inactive, and, in 8 cases, by radioimmunoassay (RIA). IL‐6 was measured as hybridoma growth factor (HGF) on the 7TDI cell line. TAM did not release appreciable levels of IL‐I spontaneously and, upon LPS stimulation, were poor producers of this monokine compared to blood monocytes. In contrast, TAM supernatants contained a high level of HGF in the absence of deliberate stimulation, and exposure to LPS either did not affect or further augmented production of this monokine. HGF activity of TAM supernatants was completely blocked by anti‐IL‐6 antibodies, Ascites fluid from 8 ovariancarcinoma patients contained high levels of HGF activity, blocked by anti‐IL‐6 antibodies. Thus, TAM exhibit a dissociation in their capacity to release the functionally related monokines IL‐I and IL‐6. IL‐6 produced by TAM may account for the elevation of liverderived acutephase proteins associated with malignancy.