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Suppression of c‐myc mRNA expression by steroid hormones in HTLV‐I‐infected T‐cell line, KH‐2
Author(s) -
Koizumi Tamio,
Nakao Yoshinobu,
Kawanishi Michiko,
Maeda Sakan,
Sugiyama Taketoshi,
Fujita Takuo
Publication year - 1989
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910440425
Subject(s) - cell culture , messenger rna , dexamethasone , microbiology and biotechnology , biology , receptor , t cell leukemia , beta (programming language) , t cell receptor , steroid hormone , alpha (finance) , leukemia , gene expression , endocrinology , t cell , human t lymphotropic virus 1 , hormone , medicine , gene , immune system , immunology , biochemistry , genetics , construct validity , nursing , computer science , patient satisfaction , programming language
Abstract The effects of l,25(OH) 2 D 3 and dexamethasone on cellular proliferation and gene expression of the HTLV‐l‐infected T‐cell line, KH‐2, established from a patient with adult T‐cell leukemia, endemic in the south‐west Japanese islands and the Caribbean, were examined. KH‐2 cells are integrated by HTLV‐I proviral DNA and expressed mRNA for c‐myc , IL‐2 receptor a‐chain (IL‐2Rα), and T‐cell receptor β‐chain (TCRβ) while it did not express IL‐2 mRNA. l,25(OH) 2 D 3 and dexamethasone did not suppress the mRNA levels of HTLV‐I, IL‐2Rα or TCRβ but reduced the c‐myc mRNA level. The reduction of c‐myc mRNA level was marked in l,25(OH) 2 D 3 ‐treated cells but relatively weak in dexamethasone‐treated cells. This inhibitory effect of the steroid hormones correlated with the inhibition of KH‐2 cell proliferation.