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Coexistence of delta sleep‐inducing peptide and serotonin in midgut carcinoid tumour cells in vivo and in vitro
Author(s) -
Ahlman H.,
Åhlund L.,
Nilsson O.,
Dahlström A.,
Bjartell A.,
Ekman R.
Publication year - 1989
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910440414
Subject(s) - serotonin , immunocytochemistry , pentagastrin , midgut , in vivo , pathology , in vitro , carcinoid syndrome , chromogranin a , biology , medicine , endocrinology , secretion , immunohistochemistry , receptor , biochemistry , botany , microbiology and biotechnology , gastric acid , larva
By immunocytochemistry, delta sleep‐inducing peptide (DSIP) was demonstrated to coexist with serotonin (5‐HT) in a majority of midgut carcinoid tumour cells studied in biopsies and long‐term cell cultures. Tumour cell colonies were characterized ultrastructurally and by confocal laser microscopy. The cultures produced several DSIP‐like peptides chromato‐graphically separated from culture media. DSIP has not yet proved to be a useful tumour marker clinically. Provocation with pentagastrin in patients with midgut carcinoid syndrome resulted in increased peripheral levels of 5HT, but not of DSIP.