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Presence on a human melanoma of multiple antigens recognized by autologous CTL
Author(s) -
Van Eynde B. Den,
Hainaut P.,
Hérin M.,
Knuth A.,
Lemoine C.,
Weynants P.,
Van Bruggen P. Der,
Fauchet R.,
Boon T.
Publication year - 1989
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910440413
Subject(s) - ctl* , antigen , melanoma , immunology , biology , clone (java method) , cytolysis , cytotoxic t cell , in vitro , cancer research , genetics , gene , cd8
We derived from blood lymphocytes of a melanoma patient a large number of cytolytic T‐cell clones directed against a cell line of the autologous tumor. Three distinct groups of antigens were recognized by these CTL on the autologous melanoma cells: group A consisted of stable antigens present on all sublines, whereas antigens B and C appeared unstable and were expressed by distinct sublines. In vitro immunoselec‐tions with various anti‐A CTL clones were applied to the melanoma cells and variants resistant to 3 different CTL clones were obtained. These variants remained sensitive to other anti‐A CTL clones, indicating that group A comprises at least 4 different antigens (D, E, F and A). From a total of 76 CTL clones obtained from lymphocytes collected from the patient at various times, we found that 45 were anti‐B, 17 were anti‐C, 2 were anti‐D, 9 were anti‐E, 2 were anti‐F and I was anti‐A′. It is therefore likely that the 6 antigens identified by these CTL clones represent all or nearly all the transplantation antigens recognized by autologous CTL on this human melanoma.