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Site‐associated expression of endogenous tumor lectins
Author(s) -
Glaves Dorothy,
Gabius HansJoachim,
Weiss Leonard
Publication year - 1989
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910440322
Subject(s) - receptor , endogeny , sialic acid , biology , biochemistry , kidney , melibiose , lectin , glycolipid , staining , microbiology and biotechnology , chemistry , maltose , sucrose , endocrinology , genetics
The expression of endogenous lectins was compared in mouse Lewis lung carcinomas growing in the kidney or sub‐cutaneously. A panel of carbohydrates coupled to a biotiny‐lated carrier molecule (bovine serum albumin), biotinylated desialylated glycoproteins and sulfated polysaccharides were used in histochemical assays to detect the presence and distribution of carbohydrate receptors. Heterogeneous staining patterns were observed with most carbohydrates in sections of tumor tissue from both anatomic sites, and staining intensities also varied within each section. At least 2 populations of cells were identified at each site, of which one had receptors for all carbohydrates, while the other had no receptors for melibiose, sialic acid, or α‐glucosides (maltose and glucose). There were quantitative differences in expression of endogenous lectins by tumors growing s.c. or in the kidney; 3LL cells growing in the kidney bound 6 out of 10 carbohydrates to a greater extent than 3LL cells in s.c. tumors. Conversely, 3LL cells in s.c. tumors bound heparin and asialofetuin to greater extents than cancer cells in kidney tumors. Biochemical analyses of detergent extracts of tissues subjected to affinity chromatography and subsequent SDS‐PAGE revealed quantitative and also qualitative differences in lectins between tumors growing in the 2 anatomic sites.