Premium
Expression of TGF‐β and procollagen type I and type III in human gastric carcinomas
Author(s) -
Yoshida Kazuhiro,
Yokozaki Hiroshi,
Niimoto Minoru,
Ito Hisao,
Ito Masanori,
Tahara Eiichi
Publication year - 1989
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910440303
Subject(s) - procollagen peptidase , messenger rna , biology , transforming growth factor , carcinoma , pathology , cell culture , cancer research , endocrinology , medicine , gene , biochemistry , genetics
Abstract To elucidate the difference between scirrhous and non‐scirrhous gastric carcinomas, we examined the expressions of TGF‐β, procollagen type I and type III in 7 gastric carcinoma cell lines and 37 gastric carcinoma tissues, and also examined the effect of TGF‐β on the expression of procollagen mRNA by TMK‐I cells. TGF‐β mRNA was detected in all the tumors examined in vivo and in vitro . Interestingly, 9 (90%) of 10 scirrhous gastric carcinomas revealed higher levels of TGF‐β mRNA than normal tissues, while 8 (38%) of 21 well‐differentiated adenocarcinomas had higher TGF‐β mRNA levels than normal tissues. As for procollagen mRNA, most of the human gastric carcinoma cell lines expressed type‐l procollagen mRNA and MKN‐I expressed type‐Ill procollagen mRNA. Furthermore, procollagen type‐l mRNA accumulation in TMK‐I cells was increased by exogenous TGF‐β. Most of the tumor tissues from surgical specimens expressed higher procollagen mRNA than normal tissues. These results indicate that TGF‐β produced by carcinoma cells might stimulate collagen synthesis not only by fibroblasts but also by carcinoma cells themselves, leading to diffuse fibrosis of scirrhous gastric carcinomas.