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Establishment of human malignant mesothelioma cell lines
Author(s) -
Versnel Marjan A.,
Bouts Monique J.,
Hoogsteden Henk C.,
Van Kwast Theodorus H. Der,
Delahaye Mick,
Hagemeijer Anne
Publication year - 1989
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910440212
Subject(s) - mesothelioma , pathology , cell culture , epidermal growth factor , autopsy , malignant cells , mesothelial cell , malignant transformation , medicine , biology , cancer research , cancer , genetics
Abstract Seventeen human malignant mesothelioma cell lines were isolated from 61 samples (46 effusions, 9 biopsies and 6 tumors obtained at autopsy) collected from patients with a confirmed malignant mesothelioma. The method used is given in detail. Cytogenetic analysis of growing cultures is the best indicator to determine whether the observed proliferation concerns malignant or normal mesothelial cells. The addition of epidermal growth factor (EGF) and hydrocortisone (HC), or EGF alone, to the culture medium increases the chances of successful isolation of a malignant mesothelioma cell line.