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Benzoyl peroxide enhances the invasive ability of a mouse epidermal carcinoma cell line
Author(s) -
Bonfil R. D.,
Momiki S.,
Conti C. J.,
KleinSzanto A. J. P.
Publication year - 1989
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910440129
Subject(s) - benzoyl peroxide , cell culture , hydrogen peroxide , peroxide , superoxide dismutase , matrigel , epidermis (zoology) , in vitro , chemistry , cancer research , squamous carcinoma , reactive oxygen species , carcinoma , microbiology and biotechnology , pathology , biology , biochemistry , medicine , oxidative stress , anatomy , organic chemistry , polymerization , genetics , polymer
The effect of the skin tumor promoter benzoyl peroxide on the invasive potential of a murine squamous carcinoma cell line was investigated using an in vitro assay based on the capacity of cells to migrate through a porous filter coated with matrigel. Pre‐treatment of the murine squamous carcinoma cell line CH72 with benzoyl peroxide added to the tissue culture medium increased the invasive capacity of these cells 5‐8 times. No effects were observed on cells from primary cultures of normal murine epidermis. The simultaneous treatment of CH72 cells with benzoyl peroxide and the free radical scavengers, superoxide dismutase or CuS0 4 , prevented the increase and the values did not differ significantly from the baseline invasive potential. This suggests that activated oxygen species participate in the benzoyl peroxide‐induced enhancement of the invasive capacity of these carcinoma cells.