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Identification by C‐banding of two human prostate tumour cell lines, 1013L and DU 145
Author(s) -
HartleyAsp Beryl,
Billström Anita,
Kruse Elisabeth
Publication year - 1989
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910440128
Subject(s) - karyotype , centromere , biology , chromosome , y chromosome , microbiology and biotechnology , chromosome analysis , genetics , marker chromosome , gene
Two human prostate carcinoma cell lines are easily distinguished by C‐banding. DU 145 has one metacentric chromosome with a large centromeric band, I metacentric and 2 acrocentric chromosomes with interstitial C‐bands. The Y chromosome was present in 48% and 42% of the cells at passages 83 and 106, respectively, whereas in I0I3L only one metacentric chromosome contained distinct extra C bands—one at the centromere and one interstitially. The Y chromosome was present in 72% of the cells at passage 15 and in 20% at passage 22, but by passage 44 it had disappeared. Karyotype analysis using G‐banding revealed that DU 145 had retained several of its original markers, and demonstrated multiple marker chromosomes in 1013L.