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Influence of shear stress on tumor‐cell adhesion to endothelial‐cell extracellular matrix and its modulation by fibronectin
Author(s) -
Bastida Eva,
Almirall Lourdes,
Bertomeu M. Cruz,
Ordinas Antonio
Publication year - 1989
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910430635
Subject(s) - fibronectin , extracellular matrix , adhesion , laminin , microbiology and biotechnology , cell adhesion , basement membrane , endothelial stem cell , cell , chemistry , extracellular , biology , in vitro , biochemistry , organic chemistry
The interaction of malignant cells with blood‐vessel endothelial cells and their underlying basement membrane is an important step in the development of secondary metastases. We investigated the interactions of highly metastatic human tumor cells, the A‐549 adenocarcinoma of the lung, with cultured endothelial cells (EC) and their extracellular matrix (ECM). We studied the adhesion patterns of the A‐549 tumor cells to EC and ECM under static and flow conditions. Our results provide evidence that tumor‐cell adhesion depends not only on the characteristics of the tumor cells themselves, but also on the properties of the EC and ECM. Our results also indicate that tumor‐cell adhesion to ECM is shear‐rate‐dependent, and that it is partially modulated by fibronectin. Moreover, our results suggest that the arg‐gly‐asp (RGD) common adhesion receptor site is also involved in the adhesion of the A‐549 cells to EC and ECM.