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Direct and serial transplantation of Ph 1 ‐positive acute lymphoblastic leukemia into nude mice
Author(s) -
Miyagi T.,
Kubonishi I.,
Ohtsuki Y.,
Ohyashiki J. H.,
Toyama K.,
Miyoshi I.
Publication year - 1989
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910430631
Subject(s) - leukemia , transplantation , chronic myelogenous leukemia , clone (java method) , cancer research , acute lymphocytic leukemia , biology , cd19 , philadelphia chromosome , microbiology and biotechnology , pathology , immunology , chromosomal translocation , medicine , flow cytometry , lymphoblastic leukemia , genetics , dna , gene
A new Philadelphia chromosome (Ph 1 ‐positive acute lymphoblastic leukemia (ALL) cell line was established in nude mice by direct and serial transplantation of peripheral blood leukemia cells from an adult patient. Although the patient's leukemia cells did not grow in vitro , they were successfully transplanted for 8 serial passages, giving rise to progressive growth of tumors with frequent involvement of lymph nodes, liver, spleen, bone marrow, and meninges. The tumor cells could also be passaged in an ascites form. This in vivo cell line, designated PALL‐1, retained the Ph 1 chromosome, t(9;22) (q34;q11), and pre‐B‐cell phenotype (SmIg − , CpIg − , CD10 + , CD19 + , OKIal + , and CD38 + ), like the original leukemia cells. Molecular genetic analysis of PALL‐1 cells revealed neither bcr rearrangement nor 8.5‐kb abl ‐related mRNA that is characteristically seen in Ph 1 ‐positive chronic myelogenous leukemia (CML). Thus, the PALL‐1 cell line is genetically distinct from CML. It may provide a useful model for an understanding of the cellular and molecular biology of Ph 1 ‐positive ALL without classical bcr rearrangement.