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Urokinase‐type plasminogen activator biosynthesis is induced by the EJ‐Ha‐ ras oncogene in CL26 mouse colon carcinoma cells
Author(s) -
Testa J. E.,
Medcalf R. L.,
Cajot J.F.,
Schleuning W.D.,
Sordat B.
Publication year - 1989
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910430513
Subject(s) - transfection , plasminogen activator , oncogene , microbiology and biotechnology , urokinase receptor , urokinase , biology , messenger rna , activator (genetics) , carcinoma , cancer research , signal transduction , endocrinology , gene , cell cycle , biochemistry , genetics
CL26 murine colon carcinoma cells express urokinase‐type plasminogen activator (u‐PA) mRNA and activity after transfection with the activated c‐Ha‐ ras ‐1 (EJ‐ ras ) oncogene cloned from the EJ bladder carcinoma. PA activity and mRNA in control cells transfected with the non‐mutated c‐Ha‐ ras ‐1 (CO‐ ras ) gene remained negative. Ras mRNA was detected in EJ‐ ras ‐ and CO‐ ras ‐transfected cells, but not in untransfected or pSV 2 ‐ neo ‐transfected cells. These results indicate that u‐PA biosynthesis can be modulated by EJ‐Ha‐ ras ‐dependent pathways of signal transduction.