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Blood group abh‐related antigens in normal and malignant bladder urothelium: Possible structural basis for the deletion of type‐2 chain abh antigens in invasive carcinomas
Author(s) -
Ørntoft Torben F.,
Wolf Hans,
Clausen Henrik,
Dabelsteen Erik,
Hakomori SenItiroh
Publication year - 1989
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910430505
Subject(s) - antigen , urothelium , biology , monoclonal antibody , antibody , pan t antigens , isoantigens , microbiology and biotechnology , immunology , endocrinology , urinary system
A complete panel of mouse monoclonal antibodies (MAbs) against Type‐2 chain (Galβ1‐4GlcNAc‐R) blood‐group antigens (N‐acetyl‐lactosamine, Le x , H, Le y , A monofucosylated, ALe y , repetitive A) was used in a detailed immunohistological study of the modulation of these carbohydrate antigens in transitional‐cell carcinomas. The histological and cellular locations of these antigens were studied in 19 normal bladder biopsies and 53 transitional‐cell carcinomas with as well as without neuraminidase treatment of tissue sections in order to uncover potential sialylated antigens. The antigen expression was correlated to individual A 1 A 2 BO, Lewis, and secretor status. Several alterations of blood group expression were found: (1) loss of A and H antigens with accumulation of Le y antigens; (2) loss of correlation between antigen expression and secretor status; (3) disruption of the orderly stratification of blood‐group antigen expression in relation to cell layers; and (4) changes in subcellular location of antigen expression. The present data indicate that deletion of Type‐2 chain ABH antigens in transitional‐cell carcinomas is associated with α1‐3 fucosylation of the H antigen leading to accumulation of Le y antigens.