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Expression of 7F7‐antigen, a human adhesion molecule identical to intercellular adhesion molecule‐1 (ICAM‐1) in human carcinomas and their stromal fibroblasts
Author(s) -
Vogetseder Werner,
Feichtinger Hans,
Schulz Thomas F.,
Schwaeble Wilhelm,
Tabaczewski Piotr,
Mitterer Manfred,
Böuck Günther,
Marth Christian,
Dapunt Otto,
Mikuz Gregor,
Dierich Manfred P.
Publication year - 1989
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910430504
Subject(s) - cell adhesion molecule , lymphokine , stromal cell , intercellular adhesion molecule 1 , biology , antigen , cell–cell interaction , microbiology and biotechnology , pathology , clone (java method) , cell culture , cancer research , immunology , medicine , genetics , dna
The 7F7‐antigen is a widespread 85‐kDa membrane adherence molecule involved in heterotypic adhesion between PHA‐blasts and fibroblasts. Immunofluorescence analysis of COS cells transfected with clone p1CAM‐1 indicated that 7F7‐antigen is identical with ICAM‐1, the ligand for the contact molecule LFA‐1. We have investigated the expression of ICAM‐1 on several human carcinomas. Tumor cells in most carcinomas, with the exception of squamous‐cell carcinomas, expressed very little ICAM‐1 or none at all. In contrast, marked expression of this molecule was observed on fibrous tissue in the vicinity of carcinoma cells, its intensity correlating with lymphatic infiltration in these tumors. We also examined the expression of ICAM‐1 on carcinoma cell lines and its induction by treatment with interferon‐gamma (IFN‐γ). The inducibility of ICAM‐1 expression on cultured fibroblasts by several lymphokines suggests that the expression of ICAM‐1 in the vicinity of carcinoma cells is effected by lymphokines produced by activated lymphocytes/macrophages within the tumor.