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Differential expression of cell adhesion molecules in variants of K1735 melanoma cells differing in metastatic capacity
Author(s) -
Linnemann Dorte,
Raz Avraham,
Bock Elisabeth
Publication year - 1989
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910430428
Subject(s) - melanoma , metastatic melanoma , cell adhesion molecule , adhesion , cell adhesion , microbiology and biotechnology , biology , expression (computer science) , cancer research , cell , chemistry , genetics , computer science , programming language , organic chemistry
We have investigated the expression of 2 neural‐cell adhesion molecules, NCAM and L1, in K1735‐C116 and ‐M1 melanoma cells which differ qualitatively in their metastatic potential, i.e. , M1 cells are metastatic whereas C116 cells are not. We have found that NCAM in C116 cells are expressed as 2 quantitatively major glycosylated polypeptides with Mr of 145,000 and 120,000 and a minor 190,000 Mr polypeptide, whereas M1 cells expressed NCAM as 3 glycosylated polypeptides with Mr of 200,000, 140,000 and 120,000. The amount of NCAM in M1 cells constituted only 60% of the amount observed in C116 cells. In C116 cells, the 145,000 and 120,000 Mr NCAM polypeptides were sulphated whereas NCAM did not appear to be sulphated in M1 cells. No phosphorylation of NCAM in the 2 cell lines was observed. L1 was expressed as a phosphorylated glycoprotein with Mr of 210,000 in M1 cells whereas no L1 expression was observed in C116 cells. L1 was not sulphated in M1 cells.