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Chromosomal assignment of woodchuck hepatitis virus (WHV) dna integration sites in a woodchuck hepatocellular carcinoma‐derived cell line (WH257GE10)
Author(s) -
Mizuno Yasutsugu,
Murakami Seishi,
Matsushita Fumiaki,
Unoura Masashi,
Kobayashi KenIchi,
Migita Shunsuke,
Hattori Nobu,
Ohno Sinsuke
Publication year - 1989
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910430419
Subject(s) - woodchuck hepatitis virus , biology , chromosome , hepatocellular carcinoma , dna , virology , hepatitis b virus , microbiology and biotechnology , virus , hepadnaviridae , genetics , gene
Abstract The chromosomal sites of woodchuck hepatitis virus (WHV) DNA integration were identified in a woodchuck hepatocellular carcinoma‐derived cell line (WH257GE10) by the in situ hybridization technique using 3 H‐labelled WHV whole genome (WHV 2) as a probe. The G‐banded chromosome spreads from WH257GE10 were identified and diagrammed schematicaiiy according to their band patterns. WHV DNA was integrated into 2 sites: 33 region of the long arm of chromosome 6 (6q33) and 31 region of the long arm of chromosome 8 (8q31). Chromosomal sites of WHV DNA integration were stable during successive passage periods analyzed in vitro .