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Suppression of transformation and immortality in human/chinese hamster fibroblast hybrids—a model for suppressor gene isolation
Author(s) -
WynfordThomas D.,
Bond J. A.,
Paterson H.
Publication year - 1989
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910430222
Subject(s) - chinese hamster , biology , fibroblast , somatic cell , genetics , hamster , microbiology and biotechnology , cell culture , gene , malignant transformation , foreskin , transformation (genetics)
Somatic cell hybrids were produced by fusion of normal human (foreskin) fibroblasts and a transformed Chinese hamster fibroblast line V79‐8. Overall, approximately 30% of hybrid clones showed stable reversion to normal morphology and growth control in vitro as shown by serum and anchorage dependence. In one‐third of these clones, senescence was observed after a number of generations similar to that required for the human fibroblast parent cells to senesce. The remainder appear to be immortal. Normal human chromosomes can therefore restore growth control with or without finite life‐span to this transformed cell. V79 cells were found to be transfectable at an efficiency compatible with detection of single‐copy gene transfer from genomic DNA. Furthermore, these cells were exceptionally sensitive to negative (“suicide”) selection. Taken together, our data suggest that the V79 line represents an ideal system for isolation of human tumour suppressor genes.