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Levels of myc protein, as analyzed by flow cytometry, correlate with cell growth potential in malignant b‐cell lymphomas
Author(s) -
Holte Harald,
Stokke Trond,
Smeland Erlend,
Watt Rosemary,
Blomhoff Heidi Kiil,
Kaalhus Olav,
Ohlsson Rolf
Publication year - 1989
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910430130
Subject(s) - propidium iodide , cell cycle , flow cytometry , biology , microbiology and biotechnology , cancer research , polyclonal antibodies , cell , cell growth , dna synthesis , lymphoma , dna , b cell , antigen , antibody , chemistry , immunology , apoptosis , biochemistry , programmed cell death
We have analyzed c‐ myc protein expression during the cell cycle in malignant B‐cell lymphomas by dual flow cytometric detection of a fluoresceinated polyclonal anti‐ myc antibody and propidium iodide which binds stochiometrically to DNA. The data obtained were correlated to other parameters of cell activation such as histopathological grading, expression of the activation antigen 4F2, light scatter (proportional to cellular volume), DNA synthesis and percentage of S‐phase cells. The c‐ myc protein level was strongly correlated to parameters of DNA synthesis/content. In addition, the oncoprotein level was largely unvarying from the late G 1 phase through the rest of the cell cycle in both malignant cells and normal purified B cells stimulated to proliferate in vitro .