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Glycosphingolipids in lectin‐resistant variants of mouse lewis lung carcinoma cells
Author(s) -
Ugorski Maciej,
Påhlsson Peter,
Dus Danuta,
Nilsson Bo
Publication year - 1989
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910430119
Subject(s) - glycolipid , exoglycosidase , glycosphingolipid , ganglioside , lectin , immunostaining , sialic acid , cell culture , microbiology and biotechnology , lewis lung carcinoma , chemistry , sialyl lewis x , biology , biochemistry , cancer research , glycoprotein , immunohistochemistry , immunology , cancer , metastasis , selectin , genetics , glycan , cell adhesion molecule
Neutral glycolipids and gangliosides from murine Lewis lung carcinoma cell line LL 2 and its lectin‐resistant variants, differing in metastatic properties, were studied by fast‐atom‐bombardment mass spectrometry (FAB‐MS), exoglycosidase treatment and an immunostaining procedure. The neutral glycolipids identified in all cell lines studied included CMH, CDH, CTH, asialo G M2 , globoside and a glycolipid with a preliminary structure of Hex‐Hexl‐4HexNAc‐Hex‐Hex‐Cer. The major gangliosides were G M3 , G M2 , G M1 and G D1a . No qualitative differences in glycosphingolipid expression were found between the metastatic cell lines (LL 2 and LL 2 AAA) and the weakly metastatic variants (LL 2 5, LL 2 8, LL 2 30 and LL 2 RCA II). Some quantitative differences were observed between the cell lines, e.g. , in the level of ganglioside‐bound sialic acid, which was not apparently correlated with the metastatic capacities.