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Effects of recombinant human tumor necrosis factor‐α on the surface phenotype and the growth of human malignant glioma cell lines
Author(s) -
Zuber P.,
Accolla R. S.,
Carrel S.,
Diserens A. C.,
de Tribolet N.
Publication year - 1988
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910420525
Subject(s) - antigen , glioma , tumor necrosis factor alpha , cytotoxic t cell , human leukocyte antigen , cell culture , biology , cytokine , clone (java method) , recombinant dna , microbiology and biotechnology , cancer research , immunology , in vitro , gene , genetics
Abstract Human malignant glioma cell lines and clones were incubated with various concentrations of recombinant human TNF‐α, either alone or in combination with recombinant human IFN‐γ. The surface expression of HLA‐ABC (class I) antigens and β 2 ‐microglobulin, was significantly enhanced by TNF‐α alone on every cell line and clone tested. After incubation with both TNF‐α and IFN‐γ, the surface expression of HLA‐ABC antigens was only slightly higher than that observed with each cytokine alone. In contrast to IFN‐γ, TNF‐α had no effect on the surface expression of HLA‐DR (class II) antigens. Moreover, the surface expression of HLA‐DR induced by IFN‐γ was unaffected by TNF‐α. The increased expression of HLA‐ABC antigens after treatment with TNF‐α or IFN‐γ correlated with increased levels of HLA‐ABC‐specific mRNA. In addition, TNF‐α, like IFN‐γ, selectively enhanced the surface expression of a tumor‐associated antigen, Me14‐D12, while it had no effect on the expression of various other surface antigens. In the absence of actinomycin D, TNF‐α exhibited no direct cytotoxic/cytostatic effect on the glioma cell lines tested. These results indicate that TNF‐α can enhance the surface expression of HLA‐ABC antigens on human glioma cells in the absence of a direct cytotoxic/cytostatic effect.