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Increased formation of unintegrated viral DNA in skin fibroblasts from patients with familial polyposis coli shortly after infection with kirsten murine sarcoma virus
Author(s) -
Miyaki Michiko,
Tanaka Kiyoko,
Sato Chicko,
Akamatsu Noriko,
Mori Takeo,
Tsuchida Nobuo
Publication year - 1988
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910420519
Subject(s) - virology , murine leukemia virus , biology , virus , rna , agarose gel electrophoresis , viral transformation , dna , sarcoma , leukemia , viral replication , helper virus , microbiology and biotechnology , gene , immunology , medicine , genetics , pathology
Skin fibroblasts from patients with familial polyposis coli (FPC) exhibit increased frequency of morphological transformation, anchorage‐independent growth, and enhanced production of viral RNA and viral particles after infection with murine sarcoma virus(murine leukemia virus), MSV(MLV). These FPC fibroblasts form greater amounts of unintegrated viral DNA as compared to normal fibroblasts during the first 4 to 24 hr after infection with Kirsten MSV(KiMLV), suggesting that increased susceptibility of FPC cells to MSV is determined at an early stage of viral transformation. Several MSV‐transformed clones of both FPC and normal fibroblasts, isolated from agarose gel, were non‐producers having integrated viral genome which was expressed into viral RNA and rescued by infection with MLV.