Premium
The glioblastoma‐derived T‐cell suppressor factor/transforming growth factor beta 2 inhibits the generation of lymphokineactivated killer (LAK) cells
Author(s) -
Kuppner Maria C.,
Hamou MarieFrance,
Bodmer Stefan,
Fontana Adriano,
De Tribolet Nicolas
Publication year - 1988
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910420416
Subject(s) - glioblastoma , suppressor , cancer research , transforming growth factor beta , beta (programming language) , cell growth , transforming growth factor , biology , immunology , medicine , microbiology and biotechnology , cancer , genetics , computer science , programming language
Glioblastoma cells release factors (G‐TsF) which inhibit T‐cell proliferation. The G‐TsF is a novel member of the transforming growth factor β family and is identical to TGFβ 2 . The effect of G‐TsF and TGFβ 2 on the induction of LAK cell activity was investigated by culturing PBL obtained from normal blood donors and brain tumour patients in varying concentrations (50–500 U/ml) of interleukin 2 (IL2) alone or IL2 plus GTsF/TGFβ 2 (I ng/ml) for 4 days. Subsequent cytolytic activity was measured against autologous and allogeneic glioblastoma targets, fresh NK‐resistant melanoma cells and K562 cells. GTsF/TGFβ 2 purified from glioblastoma cell cultures and TGFβ 2 isolated from porcine platelets significantly suppressed the generation of LAK cell activity, and the inhibitory effect could be reduced by higher concentrations of IL2. The suppressive effect of TGFβ 2 was most significant during the early stages of LAK cell generation and no inhibitory effect was seen when TGFβ 2 was added directly to the cytotoxicity assay. These results suggest that human glioblastomas may exert an inhibitory influence on the generation of an immune response in vivo through the production of G‐TsF/TGFβ 2 , and that the inhibitory effect may be modified by IL2.