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Human breast and colon carcinomas express cysteine proteinase activities with pro‐aggregating and pro‐coagulant properties
Author(s) -
Grignani Guido,
Falanga Anna,
Pacchiarini Lucia,
Alessio Maria Grazia,
Zucchella Marisa,
Fratino Pietro,
Donati Maria Benedetta
Publication year - 1988
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910420414
Subject(s) - iodoacetamide , platelet , cysteine , coagulation , thrombin , iodoacetic acid , chemistry , biochemistry , enzyme , medicine , immunology
We have investigated concomitantly the pro‐aggregating and pro‐coagulant activities of II breast and 2 colon human carcinomas. Tumor tissues, obtained at surgery, were immediately processed to prepare tumor‐cell suspensions for the study of aggregating activity and tissue extracts for the study of procoagulant capacity. Nine carcinomas (8 breast and I colon) possessed a high, dose‐dependent platelet‐aggregating activity, which was present in the cell‐free supernatant and was inhibited by HgCI 2 and iodoacetic acid, specific cysteine proteinase inhibitors, while apyrase and hirudin had no significant effect; in contrast, the other tumors did not aggregate platelets. All the tumor extracts tested from 12 carcinomas (II breast and I colon) were able to activate blood coagulation in both the presence and the absence of F VII. The activity was inhibited by HgCI 2 and iodoacetamide, while Con A was less effective. Therefore, these tumors do not aggregate platelets through the production of ADP or thrombin, nor promote blood coagulation through the production and release of tissue factor; a tumor‐associated cysteine proteinase plays a major role in both pro‐aggregating and pro‐coagulant activities.