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Tumor‐selective cytotoxic effects of murine tumor necrosis factor (TNF) and interferon‐gamma (EN‐gamma) in organ culture of B16 melanoma cells and heart tissue
Author(s) -
Mareel Marc,
Dragonetti Christian,
Tavernier Jan,
Fiers Walter
Publication year - 1988
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910420327
Subject(s) - tumor necrosis factor alpha , cytotoxic t cell , melanoma , interferon gamma , in vivo , in vitro , cell culture , tissue culture , biology , pathology , organ culture , necrosis , histology , cancer research , cytokine , microbiology and biotechnology , immunology , medicine , biochemistry , genetics
Organotypically cultured, confronting pairs of B16B16 cell clusters and fragments of embryonic chick or mouse heart, as used for the study of invasion in vitro , were treated with murine TNF plus IFN‐gamma for 4 and 7 days. This treatment selectively killed the B16B16 cells and left the heart tlssue intact as assayed by histology and by plating confronting pairs and spent medium on tissue culture substrate. Using this organ culture assay, which more closely mimics the situation in vivo , we confirmed the tumor‐selective cytotoxic effects of combined treatment with TNF and IFN‐gamma as observed in separate cultures of malignant and non‐malignant cells on “artificial” substrate.