Premium
T‐cell‐mediated inhibition of EBV‐induced B‐cell transformation: Recognition of virus particles
Author(s) -
Bejarano Maria Teresa,
Masucci Maria Grazia,
Klein George,
Klein Eva
Publication year - 1988
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910420309
Subject(s) - virus , epstein–barr virus , biology , virology , inhibitory postsynaptic potential , b cell , cell culture , antigen , cytotoxicity , viral transformation , t cell , transformation (genetics) , cell , cytotoxic t cell , microbiology and biotechnology , in vitro , antibody , immunology , immune system , gene , biochemistry , genetics , neuroscience
In order to analyze the components of EBV‐specific immunological memory that contribute to the T‐cell‐mediated inhibition of EBV‐induced B‐cell transformation, we have investigated the growth inhibitory potential of T cells cultured for 3 days with B cells exposed to transforming and nontransforming EBV preparations. T cells cultured for 3 days with EBV‐infected autologous B cells inhibited the transformation of newly infected B lymphocytes. T cells cultured with the virus, with autologous uninfected B cells, or in the presence of 10, ughl Con‐A had a significantly weaker inhibitory capacity. The inhibitory capacity was induced by co‐cultivation with B cells infected with the transforming 895–8 virus strain, with the non‐transforming P3HRI virus strain, and also with UV‐inactivated B95‐8 virus. B‐cells infected with transforming B95‐8 virus induced the strongest growth inhibitory activity. Activation of the T cells was shown by their IL‐2 production, proliferation, and generation of non‐specific cytotoxicity. These results suggest that the initial event of the cell response in EBV‐infected cultures is induced by antigens associated with the viral particles presented by B lymphocytes.