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Proliferative and/or cytotoxic activity of lymphocyte clones to autologous human melanoma
Author(s) -
Fossati Giuseppe,
Anichini Andrea,
Squarcina Paola,
Mazzocchi Arabella,
Parmiani Giorgio
Publication year - 1988
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910420216
Subject(s) - cytotoxic t cell , melanoma , immunology , lymphocyte , t lymphocyte , cytotoxicity , cancer research , medicine , biology , immune system , in vitro , genetics
Peripheral blood lymphocytes (PBL) of a patient with metastatic melanoma were cultured with autologous melanoma cells (Auto‐Me) and recombinant interleukin 2 (IL‐2) (MLTC‐PBL). Thirty‐five days later, when no cytotoxicity against Auto‐Me or K562 was detectable, MLTC‐PBL were cloned in the presence of Auto‐Me, IL‐2 (25 U/ml) and Daudi cells as feeder. Eighty‐one growing clones were simultaneously screened for proliferative and cytotoxic activity to Auto‐Me. Twenty‐two clones proliferated in the presence of Auto‐Me only, 29 in the presence of IL‐2 only and 41 in the presence of Auto‐Me. Six clones expressed both cytotoxic and proliferative activity to Auto‐Me. The phenotype of 6 proliferative clones tested was CD3 + , CD4 + , WT31 + , CD8 − , CD16 − , Leu19 − , whereas that of 2 cytotoxic‐proliferative clones tested was CD3 + , CD8 + , Leu19 + , WT31 + , CD4 − , CD16 − . Specificity analysis of proliferative response of 6 clones and of cytotoxicity of 7 clones, tested on a panel of 14 different target cells, revealed a complex pattern of reactivity, each clone expressing a peculiar specificity. Our results suggest the possibility of isolating, from melanoma patients' PBL, T‐cell clones with proliferative activity to Auto‐Me and Auto‐Me plus IL‐2, and T‐cell clones which apparently express both proliferative and cytotoxic activity to Auto‐Me.