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DNA blotting analysis of human retroviruses in cerebrospinal fluid of spastic paraparesis patients: The viruses are identical to human T‐cell leukemia virus type‐1 (HTLV‐1)
Author(s) -
Imamura Jun,
Tsujimoto Atsumi,
Ohta Yoshihiro,
Hirose Shozo,
Shimotohno Kunitada,
Miwa Masanao,
Miyoshi Isao
Publication year - 1988
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910420213
Subject(s) - provirus , tropical spastic paraparesis , virology , leukemia , restriction enzyme , retrovirus , southern blot , biology , cerebrospinal fluid , virus , feline leukemia virus , myelopathy , t cell leukemia , immunology , dna , gene , spinal cord , genetics , genome , neuroscience
The structure of the integrated provirus in cell lines established from the cerebrospinal fluid (CSF) of patients with human T‐cell leukemia virus type‐1 (HTLV‐1)‐associated myelopathy (HAM) was analyzed. The digestion patterns with 3 restriction endonucleases, Sac I, Eco RI and Pst I, of the proviruses integrated in T‐lymphoid cell lines derived from the CSF of 4 HAM patients were similar to those of HTLV‐1 from adult T‐cell leukemia (ATL) patients. Integrated proviruses in one of these cell lines derived from the CSF were further analyzed in detail with 2 more restriction enzymes, Bam HI and Sma I. The results indicate that the retrovirus found in lymphocytes of the CSF from patients with HAM are very similar, if not identical, to HTLV‐I found in the leukemic lymphocytes of ATL patients.