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New cell line from hairy‐cell leukemia: Confirmation of leukemic cell origin by karyotype and IG gene analysis
Author(s) -
Tokumine Yukihiro,
Ueda Etsuko,
Ogawa Hiroyasu,
Sugiyama Haruo,
Taniwaki Masafumi,
Abe Tatsuo,
Kanayama Yoshio,
Hashimoto Tomoko,
Inoue Ryoichi,
Machii Takashi,
Kitani Teruo
Publication year - 1988
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910420119
Subject(s) - hairy cell leukemia , microbiology and biotechnology , cell culture , hairy cell , biology , antigen , leukemia , karyotype , antibody , monoclonal antibody , monoclonal , immunology , gene , chromosome , biochemistry , genetics
A hairy‐cell leukemia (HCL) line, BNBH‐1, was established from the peripheral blood of a 40‐year‐old male patient with HCL in a relatively stable clinical phase after splenectomy. The cells have since been growing continuously for more than 2 years. Their cell surface immunoglobulin (slg) was identical with that found on the surface of freshly isolated leukemic cells, consisting of IgG‐ K . The BNBH‐1 cells were more mature than the original hairy cells in their degree of B‐cell differentiation, as reflected by a decrease in slg expression together with the appearance of some cytoplasmic lg (clg) + cells, loss of EAγ‐rosette formation and reactivity with monoclonal antibody (MAb) FMC7, and an increase in the proportion of MAb PCA‐1 + cells. The BNBH‐1 cells possessed the antigen recognized by Leu‐MS, a highly specific MAb for HCL. Epstein‐Barr virus nuclear antigen (EBNA) was present. Both the freshly isolated leukemic cells and the cell line had the 14q + involving q32 chromosomal abnormality, and their lg gene rearrangements were also identical. Following exposure to 12‐O‐tetradecanoylphorbol‐13‐acetate (TPA), both the freshly isolated leukemic cells and the BNBH‐1 cells adhered to culture dishes and extended long, thin processes, a phenomenon characteristic of HCL. These results indicate that the BNBH‐1 line was derived from the leukemic hairy cells.