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TPA enhancement of the recovery of methotrexate and N‐phosphonacetyl L‐aspartate resistant mouse 3T6 cell clones is associated with transient alterations of cell cycle progression
Author(s) -
Szallasi Arpad,
Fox Margaret,
Kinsella Anne R.
Publication year - 1988
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910420116
Subject(s) - chinese hamster , cell culture , hamster , cell cycle , cell , microbiology and biotechnology , biology , methotrexate , chemistry , biochemistry , immunology , genetics
The effects of methotrexate (MTX) and N‐phosphonacetyl L‐aspartate (PALA) with and without 12‐ O ‐tetradecanoyl‐phorbol‐13‐acetate (TPA) have been tested on the cell‐cycle traverse of mouse 3T6 and Chinese hamster V79 cell lines. MTX, whether administered with or without TPA, had very little effect on the cell cycle of the V79 Chinese hamster cell line. However, low MTX concentrations produced a significant G 1 accumulation in the mouse 3T6 cell line after 24 hr, and this was accentuated by TPA treatment. These findings parallel previous observations that TPA enhances the recovery of MTX‐ and PALA‐resistant mouse 3T6 cell clones but has little or no effect on drug‐resistant colony recovery in the V79 Chinese hamster cell line. The possibility that the increase in accumulation of cells in G 1 might permit the rescue of an increased proportion of cells due to the release of purines and pyrimidines from dying cells is discussed. Such rescue should occur irrespective of whether or not the cell line has the ability to amplify its target genes.