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Induction of tumor suppression and delayed‐type footpad reaction by transfer of lymphocytes sensitized to a xenogenized tumor variant
Author(s) -
Bianchi R.,
Romani L.,
Puccetti P.,
Fioretti M. C.
Publication year - 1988
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910420114
Subject(s) - splenocyte , immune system , immunology , lymphocyte , adoptive cell transfer , mixed lymphocyte reaction , population , in vitro , delayed hypersensitivity , lymphoma , t lymphocyte , medicine , cancer research , biology , t cell , biochemistry , environmental health
Splenocytes immune to a highly immunogenic (“xenoge‐nized”, L5178Y/DTIC) variant of a murine lymphoma exert anti‐parental‐tumor activity in a systemic adoptive transfer system, the effect being apparently associated with the Lyt‐2 − fraction of the lymphocyte population. During investigation of the mechanisms of this protection, we found that the L5178Y/DTIC tumor‐immune lymphocytes exhibited an appreciable anti‐L5178Y delayed‐type hypersensitivity (DTH) response. Enrichment of those lymphocytes in L3T4 + cells significantly enhanced the protective effect as well as the DTH reaction, whereas the use of an anti‐L3T4 but not anti‐Lyt‐2 reagent blocked both activities. In vitro , lymphocyte proliferation against L5178Y cells occurred and was apparently associated with the Lyt‐2 − fraction of a population of L5178Y/DTIC immune splenocytes.