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Tumour localization using monoclonal antibodies. H. Biodistribution of A5B7/ 111 indium chelates
Author(s) -
Barnett P.,
Buckley R. G.,
Burke P. J.,
Searle F.,
Boden J. A.
Publication year - 1988
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910410808
Subject(s) - biodistribution , conjugate , monoclonal antibody , radioimmunoassay , chelation , antibody , chemistry , microbiology and biotechnology , immunoscintigraphy , antigen , monoclonal , radioimmunotherapy , cancer research , pathology , medicine , immunology , biology , biochemistry , in vitro , mathematical analysis , mathematics , organic chemistry
The anti‐CEA antibody A5B7 has been conjugated with the chelating agents DTPA and DHDE. Radioimmunoassay and FPLC studies have shown that the conjugation procedure has not impaired the CEA binding ability of the antibody or induced aggregate formation, Biodistributions of the 111 In‐labelled conjugates have been carried out in nude mice bearing the human colon carcinoma xenograft LS174T. The results show favourable tumour‐to‐tissue ratios for the DTPA conjugate.