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Engineering antibody affinity and specificity
Author(s) -
Webster D. M.,
Roberts S.,
Cheetham J. C.,
Griest R.,
Rees A. R.
Publication year - 1988
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910410804
Subject(s) - antibody , chemistry , medicine , biology , immunology
A combination of ab initio calculations, “knowledge‐based prediction”, molecular graphics and site‐directed mutagenesis has enabled us to probe the molecular details of antibody: antigen recognition and binding and to alter the affinity and specificity of an antibody for its antigen. The significance of electrostatic hydrogen bonding, hydrophilic/hydro phobic patch matching and van der Waals interactions as well as CDR:CDR interactions are discussed in relation to the results of site‐directed mutagenesis experiments on the anti‐lysozyme antibody Gloop2. The ability to generate reconstructed antibodies, chimeric antibodies, catalytic antibodies and the use of modelled antibodies for the design of drugs is discussed.