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Simultaneous Demonstration of glia‐ and glioma‐associated antigens in human astrocytomas
Author(s) -
Bilzer T.,
Martin B.,
Stavrou D.,
Keiditsch E.
Publication year - 1988
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910410712
Subject(s) - glial fibrillary acidic protein , gfap stain , antigen , astrocyte , glioma , biology , monoclonal antibody , microbiology and biotechnology , pathology , immunohistochemistry , alkaline phosphatase , astrocytoma , immunocytochemistry , antibody , cell , cell culture , immunology , cancer research , central nervous system , biochemistry , enzyme , medicine , genetics , neuroscience
Glial flbrillary acidic protein (GFAP) and glioma‐assodated antigens (GAA) defined by monoclonal antibodies (MAbs) were demonstrated simultaneously in human astrocytoma tissue. GFAP was stained by PAP‐method, GAA were visualized by avidin‐biotin‐technique using alcaline phosphatase. In primary and secondary tumors as well as in tissue culture heterogeneity of GFAP‐ and GAA‐expression is obvious. GFAP is mostly restricted to cell processes and less marked in the perinudear space. Depending on the individual antibody, MAbs‐positive material is located either in the tumor cell plasma (MUC 8‐22) or on cell surface membranes (MUC 2‐63). There is remarkable expression of GAA in celt clusters which fail to express Gf AP. At higher magnification, 3 types of cellular reactivity are detectable: (a) cells which react only with anti‐GFAP, (b) cells which react only with anti‐GAA and (c) tells which express both, GFAP and GAA, especially those of protoplasmic astrocyte type. These celts also occur in subcutaneous tumor grafts, and may thus represent not only a reactive event, but be part of tumor cell populations.