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Dynamics of antibody transport and internalization
Author(s) -
Matzku Siegfried,
Tilgen Wolfgang,
Kalthoff Holger,
Schmiegel Wolf H.,
Brocker EvaBettina
Publication year - 1988
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910410705
Subject(s) - internalization , antibody , medicine , immunology , receptor
By following the interaction of labelled anti‐tumor antibodies with target cells, it was found that in most systems cross‐linking of externally bound antibody by anti‐IgG leads to nearly complete inter nalization, whereas excess unlabelled antibody induces fast release of radioactivity from the cell. Uptake in tumor tissue did not depend on presentation of antigen at the cell surface, as demonstrated with antibody RA96 directed against an intra‐ and inter‐cellular antigen. With respect to permeation into solid tissue, autoradiography revealed highly non‐homogeneous distribution of intact IgG, while antibody fragments tended to show a more homogeneous penetration. Small spontaneous metastases showed markedly higher accumulation than large tumor processes.