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Quantitation and localization of cellular retionol‐binding protein in squamous‐cell carcinomas of the cervix uteri and of the oral cavity
Author(s) -
Wahlberg Peter,
Fex Göran,
Biörklund Anders,
Tropé Claes,
Willén Roger
Publication year - 1988
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910410523
Subject(s) - cervix , epithelium , pathology , cell , immunohistochemistry , biology , radioimmunoassay , medicine , cancer , endocrinology , genetics
The concentration of cellular retinal‐binding protein (CRBP) was determined by radioimmunoassay in biopsies of normal muscoa and squamous‐cell carcinomas of cervix uteri form 30 women. The tumour tissues contained significantly higher concentrations of CRBP (median = 120 μg/g protein) than normal mucosa (median = 32 μg/g protein). The distribution of CRBP in normal mucosa and squamous‐cell carcinomas from cervix uteri and from oral cavity was evaluated by immunohistochemical techniques. In tissue sections of normal epithlium from the cervix uteri and the oral cavity, the maturing kerationocytes in the prickle‐cell layer were moderately or strongly stained when antiserum against CRBP was used, while the proliferating cells in the basal‐cell layer were stained only lightly if at all. Squamous‐cell carcinomas of the cervix uteri and the oral cavity presented much the same picture. The observed difference in CRBP concentration between squamous‐cell carcinomas and normal squamous‐cell mucosa may therefore be more quantitative than qualitative.