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Both N‐ ras and c‐myc are activated in the shac human stomach fibrosarcoma cell line
Author(s) -
Andeol Y.,
Nakdeux P. C.,
DayaGrosjean L.,
Brison O.,
Cebrian J.,
Suarez H.
Publication year - 1988
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910410516
Subject(s) - fibrosarcoma , ht1080 , gene , point mutation , microbiology and biotechnology , carcinogenesis , cell culture , biology , mutation , cancer research , gene product , gene expression , genetics
A transforming N‐ras gene was isolated from the SHAC human stomach fibrosarcoma cell line. A single‐point mutation resulting in the substitution of histidine for glutamine at codon 61 was found in the SHAC transforming allele. The N‐ras gene is overexpressed in the tumor cells and transformant cells. The N‐ras p21 product was studied by immunoprecipi‐tation and showed no alteration in mobility as compared to the normal p31 protein. The c‐myc gene is amplified and overexpressed in these cells. This report gives evidence that an amplified c‐myc and a mutated N‐ras gene are both present in this tumor cell line and provides support for the idea that co‐operation of at least 2 activated cellular oncogenes is required for carcinogenesis.