Premium
A colposcopical lesion of the uterine cervix frequently associated with papillomavirus type 16 as detected by in situ and southern blot hybridization: A cytohistological correlation study
Author(s) -
Morin Carol,
Bouchard Celine,
Fortier Michel,
Levesque Roger,
Meisels Alexandra
Publication year - 1988
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910410410
Subject(s) - in situ hybridization , biology , southern blot , lesion , pathology , cervix , colposcopy , immunohistochemistry , cervical cancer , cancer , medicine , gene expression , gene , genetics
A convoluted aceto‐white lesion was observed by colpos‐copy on the uterine cervix of 19 patients. A search for specific types of papillomavirus was undertaken in these lesions which proved histologically to be a vertical association of CIN in the lower strata of the squamous epithelium and typical condy‐loina in the uppermost layers. Previous cell samples correlated with histology in only (I out of the 19 cases, and were not therefore an accurate predictor of his to logic findings. However 16 out of our (9 cases had sufficient cellular changes to warrant colposcopy and biopsy. Papillomavirus caps id antigen was detected by peroxidase‐antiperoxidase staining technique in 12 out of the 19 cases (63.1%). In situ hybridization using biotinylated HPV‐16 DNA probes was positive in 13 out of 18 cases (72.2%). Southern blot hybridization gave the typical Pstl pattern of HPV‐ONA in 8 out of 9 cases examined. The detection of HPV‐DNA and HPV capsid antigen was particularly high in this “convoluted”cervical lesion, suggesting that the virus remains biologically active. It may therefore be assumed that this lesion represents a particular phase of the disease process in which early gene function and late gene expression of the viral genome are markedly manifested. Furthermore, this lesion may well represent a link between the early manifestation of HPV infection (condyloma) and CIN, which constitute a morphologic continuum initiated by papillomviruses.