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Stability of cytogenetic alterations in a human melanoma cell line and five clonal derivatives
Author(s) -
de Lucca Edmundo Jose,
Pathak Sen,
Cheung MeiChi Peng
Publication year - 1988
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910410222
Subject(s) - isochromosome , biology , karyotype , genetics , chromosome , cytogenetics , marker chromosome , microbiology and biotechnology , genetic marker , cell culture , gene
A cytogenetic study was done on a human malignant melanoma cell line and its 5 clones. Chromosome banding analysis indicated the presence of 7 “shared” markers (M) and 9 unique markers (m) that were present only in the clones. Chromosomes 1, 5, 9, 12, 17 and 21 were involved in M‐markers and chromosomes 1, 2, 4, 6, 8, 9, 11, 16, 17, 18 and 21 were involved in m‐marker formation. Both parental and clonal lines had near‐triploid chromosome numbers. A number of M‐markers were isochromosomes of the short (p) and long (q) arms of chromosome I. Our cytogenetic data indicate that the parental line contained subpopulations of cells that were in different stages of karyotypic evolution.